The National Institute of Food and Agricultures Agricultural Genome to Phenome Initiative (AG2PI) focuses oncollaborative science engagement that intends to develop a community of researchers across both crops and animalsthat will lay the foundation for expanding knowledge concerning genomes and phenomes of importance to the agriculture sector of the United States. Success of the initiative will inform approaches to understanding how variable weather, environments, and production systems interact with genetic diversity present in crops and animals to impact growth and productivity. This will provide greater accuracy in predicting crop and animal performance under variable conditions and more efficient selection of well-adapted, superior genotypes that farmers and ranchers can produce.